The Wolverhampton Wanderers footballer was pictured arriving at his home this afternoon, without any sign of Danielle in tow.
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At the weekend rumours emerged that Jamie had cheated on wife of two years Danielle, allegedly having sex with a woman while he was staying at Malmaison hotel in Birmingham.
After the infidelity was reported on Sunday, Danielle was seen driving away from the couple's home in her white Audi, and on Monday family members were seen collecting toys and belongings from the property.
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According to a friend of the former Miss England, Danielle spent Sunday grilling her husband for some answers surrounding the alleged cheating.
A blonde woman in her late 20s has claimed that she met the footballer at the Birmingham Bushwhackers club earlier in July, and that he later took her and another woman back to his hotel for a threesome.
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But after one of the women left, Jamie apparently proceeded to have sex with the blonde after confessing that he and his wife had already split up.
Jamie and Danielle have three sons together and have always appeared the devoted couple, with Danielle posting a snap just days ago of a tattoo bearing Jamie's name on her back.
'Love my new tattoo dedicated to my gorgeous hubby Jamie love him so much #forever' she captioned the black and white shot of the body art.
Danielle has maintained twitter and instagram silence since the allegations came to light.