They continue to support each other as parents and will remain friends
Tamzin, 42 and her 34 year-old husband were married in June 2006 and have two children together.
Their first daughter, Florence, was born in 2008 while her younger sister Marnie Mae Ellis was born last August.
A spokesman for Tamzin and Tom confirmed the news on Monday night, and said that the former couple would ‘remain friends.’
‘Tamzin Outhwaite and Tom Ellis are sad to confirm they have separated. They continue to support each other as parents and will remain friends,' said the statement.
'They ask for sensitivity at this difficult time,' it added.
Tom – who now stars in hit TV show Miranda – told a UK newspaper last March how his life changed when he met his future wife through their mutual friend James McAvoy.
'My life changed in 2005 on the day I met my wife, Tamzin Outhwaite' he wrote.
She was in a play called Breathing Corpses with James McAvoy, one of my best mates from drama school.
'I knew who she was and I’d fancied her quite a bit when she played Melanie Owen in EastEnders.
After spotting Tamzin in a bar a few days later, Tom revealed that they pair 'chatted together for the rest of the night.'
'I gave her a lift home and told her I’d love to see her the next day...After just a couple of weeks, I already knew she was the person I wanted to spend the rest of my life with.'